The kits are on sale in vending machines in the female toilets at the Anchor Inn at Cowes on the Isle of Wight — known as the favourite haunt of international yachtsmen during the Cowes Week regatta.
The introduction of the £5 kits has split local medical opinion in an area that for years had the country's highest teenage pregnancy rate.
Dr Mark Denman-Johnson, one of the islands leading GPs said: "If you have a youngster who suspects they are pregnant they can get the necessary full range of support if they go to their doctor's surgery.
"On its own, a pregnancy-testing kit is just one part of the service which should be provided. Contraceptive advice needs to go with it. Clearly that sort of advice is not available from your local pub."
But Dr Peter Old, director of public health on the island, said: "It is a good idea for people to have access to pregnancy testing kits when they need them. I do not see why it is a bad thing they are available in pubs."
Anchor licensee Chris Troup said: "In a town like this everyone tends to know everyone else and women may prefer the anonymity of buying a kit here as opposed to getting one from a busy chemist."