The Guild of Master Victuallers (GMV) has urged the government to scrap its review of the smoking ban over fears that it could be extended.
Bill Sharp, the GMV's chairman, has warned that "thousands of pubs are at risk from extensions to the smoking ban".
A review of the ban, which was introduced in July 2007, is due this autumn - but Sharp suggested this could lead to plans to ban smoking outside pubs and in beer gardens.
"This would be utterly devastating, especially for smaller more traditional pubs and would accelerate their decline," said Sharp.
He added: "The coalition has the opportunity to stop the review and it should take it - they have claimed to be focused on deregulation and on opening Britain for business. This is a great opportunity for them to walk their talk and relax the rules. Further regulations would a spectacular own goal politically and economically".
The GMV pointed to figures which show that 4,148 have closed since the ban came into effect - a decline of 7.6 per cent.
However, according to CGA figures, the rate of closures in the three years prior to the ban was 2.7 per cent.