The Support and Care service, which was launched at the end of 2013, aims to help up to 1,000 people from the trade every year.
The helpline is available to anyone who is working in, or retired from, the trade and provides help and guidance on issues such as dealing with difficult financial problems.
The new service is available seven days a week from 8am to 8pm.
The latest donation is part of Heineken’s commitment to promoting the service through the pubs and bars that it supplies.
Claire Matthews, corporate responsibility manager at Heineken, said: “We are very happy to make a contribution towards the delivery of the helpline.”