Big Hug Brewery, in partnership with artist agency FMLY and event organisers Taps and Tents, has launched the space as the first step of a national project.
Indie rock bands Foals and Friendly Fires will perform a DJ set alongside other famous DJs, including Daniel Avery, who have agreed to play for free to help the cause.
Named Hobo House, the space near London Waterloo station offers beer, street food and entertainment until Thursday 12 December.
It will sell beers from breweries that promote social enterprise or sustainability alongside the Big Hug Brewing range.
Brewery founder Matt Williams said it would take over different spaces across the country with pop-ups to help local charities.
Williams explained: “Hobo House is our pop-up taproom and space where people can come together to engage, eat, drink and make a difference along the way.
“We want to take over different spaces across the UK and make it our home for a while before moving on.
“Each space will be different and what works in one venue might not work in the next, but one thing they all have in common is that they will allow us to make a difference to individuals and organisations in that local area by supporting chosen charities.”
Donations will be made to the charities Only A Pavement Away, Sleep Pods Bristol and the Refugee Community Kitchen among others.
The pop-up is being hosted at 26 Leake Street, in a tunnel underneath Waterloo.