The Warrington, a flagship former Victorian hotel and pub operated by Gordon Ramsay Holdings (GRH), is discreetly being marketed for sale, M&C Report understands.
Restaurant Property, the London based property agency, is believed to be sounding out potential suitors for the Maida Vale venue, which features a Grade II listed saloon and an art nouveau interior.
The chef, who is currently on annual leave in the USA, is believed to own the freehold personally. It was acquired for around £5.2m in 2006.
The same agency has also been instructed by GRH to sell The Devonshire, the company's Chiswick-based pub that was shut in June this year.
Should the two sites be sold it would leave Ramsay with just one pub, The Narrow in East London.
Ramsay is understood to have spent £800,000 refurbishing the Warrington after he acquired what was supposed to be the launchpad for a chain of gastropubs.
The entrepreneur, who recently disposed of a £20m stake in his television production company, has been hit by the economic downturn and GRH blamed the closure of The Devonshire on trading conditions in the pub market, after it "had not been meeting expectations."
The site was first put for sale in January 2009, but was promptly taken off the market following press reports. In contrast, it had been thought that The Warrington, which is privately owned by the chef, had been his best performing pub.
A spokeswoman for GRH denied that The Warrington was for sale.
No one was available to comment from Restaurant Property.