BrewDog BottleDog outlet in Gray’s Inn Road in Kings Cross was the brewer’s first bottle shop when it opened April 2014.
BrewDog Retail MD David McDowall said: “We took the really difficult decision to close the doors to BottleDog Kings Cross in order to focus our efforts on our combined bar and bottleshop sites.
“Although a popular site with plenty of BrewDog fans, dedicated bottle shop sites sit out with our core plan."
Thanks to the loyal army of beer geeks
He added: “Investments into our other locations will continue to increase the availability of awesome take-away beer in BrewDog bars.
“For our loyal army of beer geeks, we would like to say a huge thanks for visiting the site and rest assured, there are other big plans on the horizon; new bars, epic brews and plenty more awesome projects coming your way in the next year!”
Beer fans took to Twitter to voice their upset over the closure and one user said: “Genuinely gutted about this.
“Staff were always brilliant in there and the range [was] spectacular.
“Business is business I guess, but [it is] very sad.”
The brewer came under fire in 2016 when some of its products were housed in dead animal beer bottle holders.
Furious vegans called on The Vegan Society to remove their trademark from the products due to the holders.
However, in December, BrewDog announced a £70m turnover following two successful crowdfunding initiatives.
The brewer has been consistently profitable annually since 2008 with a yearly growth rate of 69%.