Lynne D’Arcy will vacate her position at the beginning of November following nine years with the Lancashire-based company.
She took over from Brian King as managing director in 2009, following a tenure as joint managing director at Admiral Taverns.
She will be succeeded by Mark Brown, the former head of legal at Admiral Taverns, who will take up the role next month.
D’Arcy said she had enjoyed the role and was confident in the future of the company in the hands of her successor.
She said: “I have thoroughly enjoyed my time with the company and believe that I leave Trust Inns in great shape with a first-class head office and operations team. I am confident that the company will enjoy continued success with Mark at its helm.”
D’Arcy was praised for her work by the directors of Trust Inns, who said she would be leaving the company in a state where it had the chance to expand its portfolio under new leadership.
The organisation is in a strong position to acquire new properties following its disposal of several sites and a refinancing programme, directors said.
They added: “We would like to thank Lynne for her hard work and dedication to the business. She leaves with our thanks and very best wishes."
"We look forward to welcoming Mark to Trust Inns.
"He is taking over an excellent estate of public houses with the opportunity to invest in the estate and on new acquisitions.”
Four new freehold properties were acquired this year and the company is keen to build on these purchases in the coming months.