The launch of Beaverworld in Ponders End, Enfield in north London comes after Heineken bought a minority stake in Beavertown in 2018, with £40m pledged to fund the new brewery.
The new venue will deliver a maximum capacity of 500,000HL a year and to brew in 150HL batches and aims to be energy efficient.
Beavertown Brewery founder Logan Plant said: “Beaverworld has been a long time in the making and we are thrilled we have now started brewing on site in Enfield."
Plant added: “We are confident with Beaverworld, we have created a quality-driven brewery with passion and love for the craft at its core.
“Beaverworld is the answer to our dream of getting more Beavertown in the hands of the masses and we could not be more excited that it is now up and running. With Beaverworld, we are expecting to create our own lasting legacy.”
Beavertown accepted an offer by Heineken to invest an undisclosed sum to help it invest in the new brewery and visitor experience in June 2018.
On the launch of the new site, Heineken craft development director Jochen Van Esch said: “This is the culmination of many years of hard work from Plant and the whole team at Beavertown.
“Our partnership meant they could open a state-of-the-art brewery – the largest brewery in London. It means even more people can enjoy Beavertown beers in pubs and at home and we will facilitate this where we can.
“Plant and his team will be able to experiment and collaborate with other brewers and share knowledge – ultimately adding excitement to the British beer scene, value to pubs and more choice for beer lovers.”