Prices at the majority of JDW pubs have risen by 10p on average, with an extra 20p being added to drinks at London venues.
This represents an average 2% increase in the majority of JDW pubs, and 4% in pubs in and around London.
JDW spokesperson Eddie Gershon said while the company occasionally does up its drink prices, it aims to keep prices as competitive as possible.
"We believe our drinks offer still represents great value for money," he said.
One customer who noticed a price hike at a Spoons in Exmouth told DevonLive they saw a pint of Ruddles go from costing 99p to £1.49.
They said: "I went in on Monday night with £2 in cash expecting to buy a couple of cheeky Ruddles before meeting my wife when she finished her exercise class, but when I get there, I discovered the prices appeared to have all gone up.
“It is still cheaper than anywhere else around, but it did mean I had to get my card out for the second pint.”
This follows a disappointing trading period for the company at the start of the year. Like-for-like sales and total sales decreased by 11.7% and 13.3% respectively in the 25 weeks to 16 January 2022, when compared to the similar period in financial year 2020, due to “Plan B” Covid measures.
Support for Ukraine
Earlier this month, the pub chain announced it would no longer stock a Russian beer after the country invaded Ukraine. Baltika Lager, which comes from St Petersburg, will be removed from about 870 pubs around the country.
A JDW spokesperson said: "In light of the situation in Ukraine we just felt that we could not stock it anymore.
"As of today all those beers won't be available in any Wetherspoons pub and either have been or are in the process of being returned to the Carlsberg distributor.
"We are not implying in any way that anyone involved with the brewery is directly involved with what's going on but we thought in light of the current situation our pubs don't want to be serving Russian beers."