Work is now underway to convert the venue on Shaftesbury Avenue, under the Zedwell Hotel in Piccadilly Circus, into a Coyote Ugly, which was the inspiration behind the movie of the same name.
Once this has been completed on the 6,200sq ft site, which was formerly a souvenir shop, it will open its doors this spring.
The deal has been headed by chairman of the Breaking Brands group Steve Lewis, which owns the UK franchise rights to the brand, with fellow directors Nick Taplin and Caroline Wilce.
The announcement follows the opening of the first London venue in Camden Town last August.
Lewis said: “Piccadilly is one of London’s busiest areas and is ideal for a bar like Coyote Ugly.
“The history of the film and bars will appeal not only to the high number of tourists who visit the area but also those visiting the capital from across the UK.
“In addition, those working in London will be able to enjoy the lively atmosphere that has made the Coyote Ugly brand one of the most popular bars across the world.
“As a brand, we will be opening more sites in the UK and abroad and dovetails with our ambitious plans to open a lot more venues across the world.”
The brand was founded by Liliana Lovell and the first Coyote Ugly opened in the early 90s in New York.
It now boasts more than 20 bars globally and the latest venue will aim to replicate the high energy, cowboy-themed fun, guests across the world have come to enjoy.
The newest addition will hold a 3am licence, which according to the brand, is the first granted in London’s West End for more than two decades and will hold 350 people.
Coyote Ugly Piccadilly Circus will also be open from 8am for breakfast and aims to be a destination venue with food and drink packages available throughout the day.